Saudi Journal of Gastroenterology
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ORIGINAL ARTICLE
Year : 2011  |  Volume : 17  |  Issue : 1  |  Page : 30-35

Non-association of IL-12 +1188 and IFN-γ +874 polymorphisms with cytokines serum level in occult HBV infected patients


1 Department of Microbiology, Hematology and Immunology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran; Molecular-Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
2 Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3 Molecular-Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran; Department of Physiology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
4 School of Biomolecular and Biomedical Science, Eskitis Institute for Cell and Molecular Therapies, Griffith University Nathan, Queensland, Australia

Correspondence Address:
Mohammad K Arababadi
Department of Microbiology and Immunology School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1319-3767.74461

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Background/Aim: Occult hepatitis B infection (OBI) is identified as a form of hepatitis in which despite the absence of detectable HBsAg, HBV-DNA is observed in peripheral blood of patients. The main aim of this study has been to investigate the association between polymorphisms in +874 of IFN-γ and +1188 of IL-12 with their serum level in patients suffering from OBI. Materials and Methods: In this experimental study, plasma samples of 3700 blood donors were tested for the presence of hepatitis B surface antigen (HBsAg) and anti-HBc by ELISA. The HBsAg - /anti-HBc + samples were selected and screened for HBV-DNA by PCR. HBV-DNA positive samples were assigned as OBI cases and ARMS-PCR techniques were performed to examine the two known polymorphisms within IL-12 and IFN-γ. In addition, the serum levels of IL-12 and IFN-γ were also determined by ELISA. Results: Results of this study demonstrated that, 352 (9.5%) out of 3700 blood samples were HBsAg - /anti-HBc + and HBV-DNA was detected in 57/352 (16.1%) of HBsAg - /anti-HBc + samples. Our results showed that groups showed significant difference in CC allele of +1188 region of IL-12 and no difference was observed in the other evaluated genes. Our results also showed that the alleles of +1188 region of IL-12 and alleles of +874 of IFN-γ were also not associated with serum level of cytokines. Conclusion: According to the results of this study, it may be concluded that the polymorphisms in +1188 region of IL-12 and +874 region of IFN-γ would not affect the expression of both cytokines at serum level in OBI patients.


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