Saudi Journal of Gastroenterology
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LETTER TO THE EDITOR  
Year : 2011  |  Volume : 17  |  Issue : 4  |  Page : 297
SMAD4 promoter hypermethylation in Kashmiri colorectal cancer cases


1 Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir; Department of Clinical Biochemistry, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India
2 Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir, India

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Date of Web Publication30-Jul-2011
 

How to cite this article:
Sameer AS, Siddiqi MA. SMAD4 promoter hypermethylation in Kashmiri colorectal cancer cases. Saudi J Gastroenterol 2011;17:297

How to cite this URL:
Sameer AS, Siddiqi MA. SMAD4 promoter hypermethylation in Kashmiri colorectal cancer cases. Saudi J Gastroenterol [serial online] 2011 [cited 2019 Jul 17];17:297. Available from: http://www.saudijgastro.com/text.asp?2011/17/4/297/82591


Sir,

Colorectal cancer (CRC) is the fourth most common cancer in men and the third most common cancer in women and a major cause of mortality and morbidity worldwide. In Kashmir valley CRC represents the third most common GIT cancer after esophageal and gastric. [1]

During the last decade, epigenetic changes have been reported in many cancers and they are now recognized to be at least as common as genetic changes. [2] Aberrant methylation of cytosine located within the dinucleotide CpG in the promoter region is by far the best categorized epigenetic change. A CpG island methylator phenotype (CIMP+) has been described in colorectal cancer (CRC) and is characterized by simultaneous methylation of multiple genes such as the cell cycle (RB, p15INK4b, p16INK4a), the TP53 pathway (p14ARF), the WNT signalling pathway (APC, E-cadherin), DNA repair (MGMT, hMLH1, BRCA1), apoptosis (DAPK), and the metastasizing process (E-cadherin, TIMP3). [3]

Inactivation of tumor suppressor genes by promoter hypermethylation has been recognized to be at least as common as gene disruption by mutation in tumorigenesis. A number of studies on colorectal cancer around the globe have demonstrated the role of promoter hypermethylation of number of different genes in development and progression of colorectal carcinoma. Likewise, we carried out this study to know the status of SMAD4 promoter methylation in Kashmiri Colorectal Cancer cases. We carried out the study on 86 colorectal cancer cases who attended Department of General Surgery for resective surgery treatment. The SMAD4 promoter lacks typical TATA boxes and CpG islands, but contains some TATA-like structures (TAAAAT) as well as some binding sites for transcription factors. We used the previously described protocol for promoter hypermethylation detection using the two restriction enzymes HpaII and MspI for differential digestion. [4] This protocol utilizes the ability of the HpaII restriction enzyme to distinguish CpG sites that are methylated versus those that are non-methylated. If the restriction sites are methylated, the methylation-sensitive HpaII does not cleave the DNA if its restriction sites are methylated but MspI is capable of cleaving the methylated restriction sites located well with the CpG islands of the promoter region [Figure 1]. Our results showed that none of the CRC cases had hypermethylation in the SMAD4 promoter region. These results were in tune with the previous study. [4] Hence, we conclude that hypermethylation is not the foremost aberration in SMAD4 gene and hence does not play any role in CRC tumorigenesis in Kashmiri population.
Figure 1: Representative picture showing the hypermethylation status of the SMAD4 promoter Lane Ma: 50bp molecular marker; Lane Mb: 100bp molecular marker; Lane 1,3,5 and 7: Amplicon of the undigested template; Lane 2,4,6 and 8: Amplicon of the HpaII

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   References Top

1.Sameer AS, Shah ZA, Syeed N, Banday MZ, Bashir SM, Bhat BA, et al. TP53 Pro47Ser and Arg72Pro polymorphisms and colorectal cancer predisposition in an ethnic Kashmiri population. Genet Mol Res 2010;9:651-60.  Back to cited text no. 1
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2.Jones PA, Baylin SB. The fundamental role of epigenetic events in cancer. Nat Rev Genet 2002;3:415-28.  Back to cited text no. 2
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3.Toyota M, Ahuja N, Ohe-Toyota M, Herman JG, Baylin SB, Issa JP. CpG island methylator phenotype in colorectal cancer. Proc Natl Acad Sci USA 1999;96:8681-6.  Back to cited text no. 3
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4.Roth S, Laiho P, Salovaara R, Launonen V, Aaltonen LA. No SMAD4 hypermethylation in colorectal cancer. Br J Cancer 2000;83:1015-9.  Back to cited text no. 4
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Correspondence Address:
Mushtaq A Siddiqi
Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1319-3767.82591

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