Saudi Journal of Gastroenterology
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ORIGINAL ARTICLE
Year : 2012  |  Volume : 18  |  Issue : 4  |  Page : 263-267

Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver


1 National Center of Excellence in Molecular Biology, Punjab University Lahore, Pakistan
2 Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Pakistan

Correspondence Address:
Gibran Ali
National Center of Excellence in Molecular Biology, Punjab University Lahore
Pakistan
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Source of Support: Research grants from Higher Education Commission (HEC) of Pakistan, Conflict of Interest: None


DOI: 10.4103/1319-3767.98433

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Background/Aim: We investigated the effect of bone marrow-derived stem cell (BMSC) transplantation on carbon tetrachloride (CCl 4 )-induced liver fibrosis. Patients and Methods: BMSCs of green fluorescent protein (GFP) mice were transplanted into 4-week CCl 4 -treated C57BL/6 mice directly to the liver, and the mice were treated for 4 more weeks with CCl 4 (total, 8 weeks). After sacrificing the animals, quantitative data of percentage fibrosis area and the number of cells expressing albumin was obtained. One-way analysis of variance was applied to calculate the significance of the data. Results: GFP expressing cells clearly indicated migrated BMSCs with strong expression of albumin after 28 days post-transplantation shown by anti-albumin antibody. Double fluorescent immunohistochemistry showed reduced expression of αSMA on GFP-positive cells. Four weeks after BMSC transplantation, mice had significantly reduced liver fibrosis as compared with that of mice treated with CCl 4 assessed by Sirius red staining. Conclusion: Mice with BMSC transplantation with continuous CCl 4 injection had reduced liver fibrosis and a significantly improved expression of albumin compared with mice treated with CCl 4 alone. These findings strengthen the concept of cellular therapy in liver fibrosis.


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