Saudi Journal of Gastroenterology
Home About us Instructions Submission Subscribe Advertise Contact Login    Print this page  Email this page Small font sizeDefault font sizeIncrease font size 
Users Online: 239 
Year : 2015  |  Volume : 21  |  Issue : 5  |  Page : 284-289

Protective effects of thymoquinone and melatonin on intestinal ischemia–reperfusion injury

1 Department of Anatomy, Gaziosmanpasa University, Faculty of Medicine, Tokat, Turkey
2 Department of Emergency Medicine, Gaziosmanpasa University, Faculty of Medicine, Tokat, Turkey
3 Department of Biochemistry, Katip Celebi University, Faculty of Medicine, İzmir, Turkey
4 Department of Histology and Embrylogy, Firat University, Faculty of Medicine, Elazig, Turkey
5 Department of Pediatric Surgery, Gaziosmanpasa University, Faculty of Medicine, Tokat, Turkey
6 Deoartment of Anatomy, Sifa University, Faculty of Medicine, Izmir, Turkey

Correspondence Address:
Assoc. prof. Ufuk Tas
Gaziosmanpasa University, Faculty of Medicine, Department of Anatomy, 60000 Tokat
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/1319-3767.166203

Rights and Permissions

Background/Aim: In the present study, we aimed to compare the potential protective effects of thymoquinone and melatonin by using equivalent dose, on oxidative stress-induced ischemia–reperfusion (IR) injury in the intestinal tissue of rats. Materials and Methods: The study was performed using 32 male Wistar–Albino rats (weighing 180–200 g) randomly divided into four groups: Group I, sham group; Group II, IR group; Group III, IR with melatonin group; and Group IV, IR with thymoquinone group. After laparotomy, ischemia and reperfusion were performed for 60 and 120 min, respectively, on all the groups. Intestinal tissue sections were stained using routine histological methods and examined under the light microscope. In addition, the sections were immunohistochemically stained using the TUNEL method for determination of apoptosis. Superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity, and malondialdehyde (MDA) levels in the intestinal tissue were also measured. Results: The IR group had significantly elevated tissue SOD activity, GSH-Px activity, and MDA levels compared with the sham group. Administration of thymoquinone and melatonin efficiently reduced these increases. Statistically significant number of apoptotic cells was observed in the intestinal tissue of IR group rats compared with the sham group. Treatment with thymoquinone and melatonin markedly reduced the number of apoptotic cells. Conclusion: The effects of melatonin and thymoquinone on IR-induced oxidative stress in rat intestines were similar. Our findings suggest that melatonin and thymoquinone protect against IR-induced injury to intestinal tissues.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded419    
    Comments [Add]    

Recommend this journal