Bacterial dysbiosis predicts the diagnosis of Crohn's disease in Saudi children El Mouzan MI, Winter HS, Al Sarkhy AA, Korolev K, Menon R, Assiri AA, - J Indian Prosthodont Soc

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Bacterial dysbiosis predicts the diagnosis of Crohn's disease in Saudi children Mohammad I El Mouzan¹, Harland S Winter², Ahmed A Al Sarkhy¹, Kirill Korolev³, Rajita Menon⁴, Asaad A Assiri⁵ ¹ Department of Pediatrics, Gastroenterology Division, King Saud University, Riyadh, Kingdom of Saudi Arabia ² Mass General Hospital for Children, Gastroenterology, MA, USA ³ Department of Physics, Bioinformatics Program, Boston University, MA, USA ⁴ Department of Physics, Boston University, Boston, MA, Boston, USA ⁵ Department of Pediatrics, Gastroenterology Division, Supervisor, Prince Abdullah Bin Khalid Celiac Disease Research Chair, King Saud University, Riyadh, Kingdom of Saudi Arabia Correspondence Address: Mohammad I El Mouzan, Department of Pediatrics, King Saud University, P O Box 2925, Riyadh 11461 Kingdom of Saudi Arabia Source of Support: None, Conflict of Interest: None DOI: 10.4103/sjg.SJG_409_20

Background: Studies have reached different conclusions regarding the accuracy of dysbiosis in predicting the diagnosis of Crohn's disease (CD). The aim of this report is to assess the utility of mucosal and fecal microbial dysbiosis as predictors in the diagnosis of this condition in Saudi children. Methods: Tissue and fecal samples were collected prospectively from children with final diagnosis of CD and from controls. Bacterial DNA was extracted and sequenced using Illumina MiSeq chemistry. The abundance and diversity of bacteria in tissue and fecal samples were determined in relation to controls. Sparse logistic regression was calculated to predict the diagnosis of CD based on subject's microbiota profile. Results: There were 17 children with CD and 18 controls. All children were Saudis. The median age was 13.9 and 16.3 years for children with CD and controls respectively. Sex distribution showed that 11/17 (65%) of the CD and 12/18 (67%) of the control subjects were boys. The mean area under the curve (AUC) was significantly higher in stool (AUC = 0.97 ± 0.029) than in tissue samples (AUC = 0.83 ±0.055) (P < 0.001). Conclusions: We found high AUC in mucosal and fecal samples. The higher AUC for fecal samples suggests higher accuracy in predicting the diagnosis of CD.

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    -  El Mouzan MI
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    -  Al Sarkhy AA
    -  Korolev K
    -  Menon R
    -  Assiri AA

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