Saudi Journal of Gastroenterology

ORIGINAL ARTICLE
Year
: 2007  |  Volume : 13  |  Issue : 4  |  Page : 172--175

The effect of chronic consumption of paraoxon on basal and pentagastrin-stimulated gastric acid and pepsin secretion in rats


Fatemeh N Rafsanjani1, Zakieh V Ardakani1, Jalal Vahedian2, Mehran Moradi3, Amir F Attar3 
1 Tehran University of Medical Sciences and Health Services, Tehran, Iran
2 Rassul-Akram Hospital and Research Center, Iran University of Medical Sciences and Health Services, Tehran, Iran
3 Baghiatalah University of Medical Sciences, Tehran, Iran

Correspondence Address:
Fatemeh N Rafsanjani
16 Azar Street, Tehran University of Medical Sciences and Health Services, Tehran
Iran

Background/Aims: Paraoxon, an organophosphate metabolite of the insecticide parathion inhibits the enzyme, acetylcholinesterase (Achase). Organophosphates affect the heart, visual system, nervous system and muscles. In this present study, we investigate the effects of the chronic consumption of paraoxon on gastric acid and pepsin secretion in N-mari rats. Materials and Methods: This study was performed from April 2003 to May 2004 in the Physiology department of Baghiatalah University of Medical Sciences, Tehran, Iran. It was performed on three groups of female N-mari rats (10 /group) each weighing 200-250 g. The first group received 0.05 mg/kg/day paraoxon subcutaneously for one month. The second group received the same chronic doses of ethyl alcohol (96%) (solvent of paraoxon) and the third group (control) received no drugs. After tracheostomy and laparatomy, gastric secretions were collected with a tube via the duodenum. Pentagastrin (25 g/kg, i.p.) was used as a gastric stimulator. Acid and pepsin secretions were measured by titration and the Anson method, respectively. The stages of the measurements were basal (first and second), stimulated and returned-basal. Results: Basal acid secretion in the paraoxon group was greater than those in the alcohol and control groups (14.61 1.46, 7.18 0.28 and 7.88 0.26 mol/15 min, respectively, P < 0.001)). Although pentagastrin-stimulated acid secretion in all the three groups was greater than that of the basal state, there were no significant differences among the three groups. Basal pepsin secretions in the paraoxon group were greater than those in the alcohol and control groups (2.97 0.32, 1.19 0.25 and 0.55 0.06 g/15 min, respectively). Pentagastrin-stimulated pepsin secretion in the paraoxon group was significantly greater than those in the alcohol and control groups (3.22 0.38, 2.22 0.46 and 1.09 0.66 g/15 min, respectively, P < 0.001). Conclusion: Chronic exposure to paraoxon results in the increased secretion of gastric acid and pepsin.


How to cite this article:
Rafsanjani FN, Ardakani ZV, Vahedian J, Moradi M, Attar AF. The effect of chronic consumption of paraoxon on basal and pentagastrin-stimulated gastric acid and pepsin secretion in rats.Saudi J Gastroenterol 2007;13:172-175


How to cite this URL:
Rafsanjani FN, Ardakani ZV, Vahedian J, Moradi M, Attar AF. The effect of chronic consumption of paraoxon on basal and pentagastrin-stimulated gastric acid and pepsin secretion in rats. Saudi J Gastroenterol [serial online] 2007 [cited 2022 Jan 20 ];13:172-175
Available from: https://www.saudijgastro.com/article.asp?issn=1319-3767;year=2007;volume=13;issue=4;spage=172;epage=175;aulast=Rafsanjani;type=0